Brightfield microscopes are standard microscopes that make use of light which travels from a source below the slide through the specimen in order to magnify it for the user to see. Brightfield or bright field microscopes are among the most well known viewing equipment in microbiology and most widely used observation mode in optical microscopy for the past three hundred years.
Brightfield microscopy is ideal for fixed, stained objects or other types of samples that possess the ability to absorb huge amount of visible light in a natural way. The images that have been produced with the use of bright field illumination turns to be dark or highly colored against a bright background such as light gray and white.
Basic compound light microscope that uses bright field microscopy obtains its light from below focusing in a brightfield light condenser. Compound microscopes that have better quality do have bright field light condenser around rack and pinion gears and a Koehler field diaphragm. These are used in order to help the light to properly focus on the specimen being examined. It is crucial to acquire an illumination accurate for the specimen when it comes to obtaining optimal Koehler illumination. There might be simple filters inserted into the light path however, it is still considered as a brightfield microscope. Techniques being used in brightfield microscopy significantly vary from those used in other types of microscopy applications.
Darkfield microscopes have the ability to manipulate light source by means of placing a disc diaphragm within the light path. These scopes can also illuminate the object by scattered light. A fluorescence microscope make use of light that includes fluoresced from the specimen and not from the original excitation light source. While a polarizing light microscope used to take the original light from the microscope bulb that passes through a polarized filter and then through a second filter called 'analyzer.'
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